Fig. 4. A multiplex PCR assay for distinguishing CMS and maintainer lines of rice. PCR was performed by multiplexing cms primers with RG136 primers. M indicates a kilobase DNA ladder as a molecular weight marker. Lanes 1 and 14: IR58025A and B; Lanes 2 and 15: IR62829A and B; Lanes 3 and 16: IR68888A and B; Lanes 4 and 17: IR68897A and B; Lanes 5 and 18: CRMS31A and B; Lanes 6 and 19: DRR2A and B; Lanes 7 and 20: Pusa5A and B; Lanes 8 and 21: PMS8A and B; Lanes 9 and 22: PMS10A and B; Lanes 10 and 23: PMS11A and B; Lanes 11 and 24: PMS12A and B; Lanes 12 and 25: DRR3A and B; Lanes 13 and 26: IR68886A and B.