Fig. 3. A PCR assay using sequence specific primers to distinguish WA-CMS and maintainer lines of rice. PCR was performed with cms primers. Lane 1: kilobase DNA marker; Lane 2: IR58025A (CMS); Lane 3: DRRH1 (hybrid); Lane 4: IR58025B (maintainer). PCR product was obtained only when template DNA is from the CMS line and hybrid.