Fig. 1. PCR amplification of a DNA sequence specific to CMS lines of rice. PCR was performed with RM9 microsatellite primers. Lane 1 contains a DNA molecular weight marker ( DNA digested by HindIII); Lane 2 contains PCR amplified product of a maintainer line (IR58025B); Lane 3, the hybrid (DRR H1), and Lane 4, CMS line (IR58025A). An extra DNA band, which is only present in the hybrid and CMS line but absent in the maintainer line, is indicated by the arrow.