Fig. 5. Southern blot analysis on T₂ individuals derived from marker-free T₁ seed of line 420-5. Upper panel, 6 desaturase probe. Lower panel, bar gene probe. Lanes 1-6, T₂ individuals from marker-free population of line 420-5. Lane 7, wild-type (WT) soybean. Lane 8, 50 pg pPTN331 positive control. DNAs digested with EcoRI. Numbers above the column refer to the percentage of GLA in the T₂ seed sample. HS indicates all individuals were scored herbicide sensitive.