Fig. 3. Southern blot analysis on line 400-1. Upper panel, 6-desaturase probe. Lower panel, bar gene probe. Lane 1, parental (T₀). Lanes 2 to 4, derived T₁ individuals. Lane 5 (WT), wild-type soybean DNA. Lane 6 (C), 50 pg pPTN331 (see Fig. 1). DNAs digested with HindIII. Designation HT above the lane indicates the individual was herbicide tolerant.