Fig. 2. Polymorphism shown by RAPD analysis, primer G-17, in parental material. The arrow indicates a fragment used for cloning and sequencing to develop the SCAR marker. The DNA ladder (Promega Corp., Madison, WI) on right consists of 11 fragments that range in size from 100 to 1000 bp in 100-bp increments, plus an additional fragment at 1500 bp. The 500-bp fragment, present at increased intensity, is indicated by an arrow.