Fig. 5. GmSUB1 expression in soybean. (A) Relative GmSUB1 mRNA levels in uninfected roots, SCN infected roots, and leaves of PI 437654 (resistant) and Williams 82 (susceptible) were determined by quantitative real-time PCR using gene-specific primers and normalizing to 18S rRNA values. Quantitation is based on C_T value as described in Materials and Methods. (B) Representative data set from quantitative real-time PCR analysis with GmSUB1 and 18S gene-specific primers. Inset panel shows standard curve graph for GmSUB1 (top) and 18S (bottom).