Fig. 1. Polymerase chain reaction amplification of pearl millet genomic DNA from 15 lines. Lanes: 1 = 81B, 2 = 94M59464B CBR, 3 = 293B, 4 = 51735B, 5 = 59025B, 6 = 086R1, 7 = Bmr eB, 8 = PI 164421, 9 = PI 286892, 10 = PI 536327, 11 = PI 307713, 12 = PI 511036, 13 = PI 561619, 14 = PI 583799, 15 = Tift 85DB, and lane M contains a 50-bp size marker (Promega Corp., Madison, WI). Two microsatellites, (A) PSM 2202 (Qi et al., 2001) and (B) CTM9, were assayed. The DNA samples were fractionated in 12% nondenaturing acrylamide gels stained with ethidum bromide.