Fig. 6. Southern blot analysis of the B-group T₁ plants. Genomic DNA (10 µg) was digested with EcoRI to release the 2.5- or 1.9-kb fragment of the sorghum omt antisense expression cassette (full-length or 5'-fragment of the sorghum cDNA, respectively) and hybridized with a sorghum omt cDNA probe. The sorghum omt antisense expression cassette includes part of the Ubi-1 promoter first intron region (about 600 bp), sorghum antisense omt (full-length or 5'-fragment of the sorghum omt cDNA), and nos terminator (see Fig. 1). -DNA (HindIII) and X174 (HaeIII) were used as DNA size markers. Bm designations indicate the T₀ parent had brown midrib phenotype. Families were generated from independent events.