Click on image to view larger version.



Fig. 5. OMT activity of the B-group T1 plants sampled at the flag-leaf stage. Plants were initiated using seed from 10 self-pollinated primary transgenic plants selected on the basis of reduced family OMT activity (Fig. 3). Family members are designated B1 through B6. The young flag leaves from transgenic and control plants of these 7-wk-old plants were assayed for OMT activity. The mean of 10 control plants was considered as 100% (0.32 pkat/mg protein). A bm in the plant designation number under the graph indicates the T0 parent had a brown midrib phenotype. Bm17-B3 could be viewed as a control as it lost the antisense omt. Families are generated from independent events, except that families bm8 and bm9 are not independent.