Fig. 1. Schematic representation of an antisense sorghum omt vector used for maize transformation. The vector contained the CaMV 35S promoter to drive bar with the nos terminator; and the maize Ubi-1 promoter (plus first intron and first exon) to drive the sorghum omt full-length cDNA (1600 bp), or the 995-bp 5'-fragment, in the antisense orientation with the nos terminator. Restriction sites labeled are; H = HindIII, B = BamHI, and E = EcoRI. Digestion with EcoRI releases a 2.5-kb fragment with the full-length omt or a 1.9-kb fragment with the 995-bp omt vectors.