Fig. 4. Ethidium bromide stained 1.5% (w/v) agrose gel showing undigested fragments of the Yr5 NIL and AVS and DpnII-digested fragments of the Yr5 NIL, AVS, and homozygous resistant (R), heterozygous (H), and homozygous susceptible (S) BC₇:F₃ lines amplified with the STS primers, Yr5STS-9 (5'-AAAGAATACTTTAATGAA-3') and Yr5STS-10 (5'-CAAACTTATCAGGATTAC-3'). M stands for 1-kp plus DNA size marker (Gibco BRL, Rockville, MD).