Fig. 3. Silver stained polyacrylamide gel showing association of the codominant markers amplified with STS primers Yr5STS-7 (5'-GTACAATTCACCTAGAGT-3') and Yr5STS-8 (5'-GCAAGTTTTCTCCCTATT-3') with the Yr5 locus. The higher band (478 bp) was present in the resistant parent (the Yr5 NIL), the homozygous resistant BC₇:F₃ lines, and the heterozygous BC₇:F₃ lines. The lower band (472 bp) was present in the susceptible parent (AVS), the homozygous susceptible BC₇:F₃ lines, and the heterozygous BC₇:F₃ lines. Phenotypic reactions of the parents and the progeny were evaluated with races PST-29 and PST-43 of Puccinia striiformis f. sp. tritici.