Fig. 3. Restriction enzyme digestions of phage DNA from 12 genomic clones (A). Each clone was digested with BamHI, EcoRI, or BamHI + EcoRI in duplicate lanes for each enzyme digestion. Lane 1, 40, 41 and 80: HindIII; lane 38 and 60: 1-kb ladder. Lane 2–7: clone 8-1; lane 8–13: clone 9-1; lane 14–19: clone 9-2; lane 20–25: clone 10-1; lane 26–31: clone 14-1; lane 32–37: clone 14-2; lane 42–47: 15-1; lane 48–53: 16-1; lane 54–59: 17-2; lane 61–66: 18-1; lane 67–72: 19-1; lane73–75: clone 21-1. The Southern blot was hybridized with pooled first strand cDNA derived from mRNA, the signals (B) from five identified genes including sugarcane tubulin (STUB), sugarcane ubiquitin (SUbi), sugarcane elongation factor (SEF1), sugarcane aquaporin (SAQ), sugarcane proline-rich protein (SPRP1) are marked by arrows.