Fig. 2. Composite autoradiograph image of restriction fragment length polymorphism (RFLP) marker 3026. Molecular weight marker is shown to left, lane numbers labeled on top, and RFLP allele positions shown on right of image. Plant identity and interpreted genotypes are as follows: Lane 1Moroccan male parent (G31276), BBDD; Lane 2autogamous female parent (AG-S4), AACC; Lane 3AG-S4 x G31276 F1 hybrid, ABCD; Lanes 4 to 24F2 progeny, AADD, ABCD, ABDD, ACCD, ABCD, AACD, BBCD, ABBC, ABCC, ABCD, ABDD, ABCD, AABD, ABCC, AABD, BBDD, AACD, AABB, ACCD, CCDD, ABCD. Note that each band does not give equal autoradiographic intensity when present as a single allele (A > B
C
D) and that genotypes are interpreted from intensity ratios relative to each other. Each RFLP allele has a double-banded pattern. Both bands of the A and C alleles are next to each other and the B and D alleles have a shared second band labeled in parenthesis as B,D near the top of the image.