Fig. 2. Banding pattern of SCAR primer pairs Creep700F/R(a) and Col400F/R(b) amplifying a single and reproducible band specifically for creeping and colonial bentgrass species, respectively. Optimal annealing temperature is at 65°C for both sets of SCAR primers. Bentgrass plants indicated are three velvet plants of cultivar EVM 99 (Lanes 1 and 2), 7 creeping plants of Cato (Lanes 3–9), and five colonial plants of 9F7 (Lanes 10–14). M indicates 100-bp marker with bright middle band indicating 600 bp.