Fig. 5. Seasonal changes in SDS-PAGE profiles of soluble proteins in roots of alfalfa cultivar AC Caribou harvested twice in the summer (A), or harvested a third time after the second harvest at 400 GDD (B), 500 GDD (C), or 600 GDD (D). Lane 1: 2nd harvest, 7 Aug. 1997; Lane 2: 3rd harvests, 8 Sept. 1997 (B), 17 Sept. 1997 (C), and 7 Oct. 1997 (D); Lane 3: 10 Nov. 1997; Lane 4: 12 Jan. 1998; Lanes 5: 11 Mar. 1998. Molecular weight standards listed to the left are 97.4, 66.2, 45, 31, 21.5, and 14.4 kDa.