Fig. 9. Southern analysis of transgenic T₁ plants. A total of 5 to 7 µg of plant genomic DNA and 30 pg of plasmid DNA were digested with EcoRV and hybridized with the same enzyme-digested plasmid DNA fragment. The arrow marks the expected 3.8-kilobase hybridizing band corresponding to Xa21 transgene in T₀ and T₁ plants. T₀ = primary transgenic plant, T₁ = selfed progenies of T13 (T₀).