Fig. 8. Southern analysis of transgenic T₀ plants. The arrow represents the expected 3.8-kilobase (kb) band corresponding to the Xa21 transgene. Lanes 1 to 5 represent nontransformed IR50, transgenic T₀ IR50-13, 14, and 15, and plasmid pC822, respectively. Five- to 7-µg plant genomic DNA and 30 pg of plasmid DNA were digested with EcoRV. The 1.4-kb polymerase chain reaction amplified fragment of pC822 was labeled with -[³²P]-dCTP using the Rediprime Labeling Kit (Amersham Heights, IL) and was used as the hybridization probe.