Fig. 3. The generation of sequence-specific amplicon polymorphism between resistant (C101A51) and susceptible (IR50) rice varieties for marker-aided selection. (A) Gel analysis of polymerase chain reaction (PCR) products before restriction digestion. (B) Gel analysis of PCR products after digestion with restriction enzyme HaeIII. Lanes 1 and 2 represent the susceptible parent IR50 and donor resistant line C101A51, respectively. The molecular weight of PCR products as banding patterns is indicated in kb (kilobases). M = Molecular weight marker (1-kb ladder).