Fig. 3. Gel-blot DNA hybridization of a positive clone (UNL12) on genomic DNA of group 1 nullisomic-tetrasomic lines, 1BS-4 and 1BS-19. Lane 1, Nulli 1A-Tetra 1B; Lane 2, Nulli 1B-Tetra 1D; Lane 3, Nulli 1D-Tetra 1A; Lane 4, 1BS-4; Lane 5, 1BS-19. Arrow represents B fragment band present in 1BS-4 and missing in 1BS-19.