Fig. 5. Proton-nuclear magnetic resonance spectrometry (¹H-NMR) analysis of alfalfa leaf extracts for poly-ß-hydroxybutyrate (PHB). (A) 500-MHz ¹H-NMR spectrum of PHB standard; (B) Line 864 transformed with phbABC genes; (C) Control, untransformed alfalfa. Note the expanded spectrum containing PHB peaks (2.5 and 5.2 ppm) present in transgenic plants.