Fig. 1. DNA Southern blot analysis of transgenic alfalfa plants. Restriction enzyme-digested genomic DNA was hybridized with ³²P-labeled phbA (Panel B) or phbB (Panel C). (A) Restriction map of the T-DNA from pMON25948. Bars underneath the map indicate sizes of restriction fragments containing phbA or phbB. R1, EcoR1 site; RV, EcoRV site; RB and LB, right and left T-DNA border, respectively; P-35S, CaMV 35S promoter; SS-TP, transit peptide of the small subunit of Rubisco. E9-3' and nos 3', terminator region of soybean E9 and Agrobacterium nopaline synthase, respectively. (B and C) Lane P, EcoRV-digested pMON25948; Lane 1, Line 863 digested with EcoR1; Lane 2, Line 863 digested with EcoRV; Lane 3, Line 864 digested with EcoR1; Lane 4, Line 864 digested with EcoRV; Lane 5, Line 916 digested with EcoR1; Lane 6, Line 916 digested with EcoRV; Lane 7, untransformed control digested with EcoR1; Lane 8, untransformed control digested with EcoRV.