Fig. 2. Changes in carbohydrate composition in crowns of three annual bluegrass ecotypes cold acclimated to the following conditions: nonhardened (NH); hardened 2 wk at 2°C (H2); hardened 2 wk at 2°C followed by 2 wk at -2°C (HF); hardened 4 wk at 2°C (H4)