Fig. 4 Southern blot analysis of pat gene in R0 and R1 transgenic plants of Cocodrie. Lanes 1–3: plasmid pPAT63 digested with EcoRI, equivalent to 1, 5, and 10 copies of the pat gene per rice haploid genome. Lanes 4–14, genomic DNAs digested with EcoRI; Lane 4: untransformed Cocodrie; Lanes 5–10: six R1 progeny plants 16-1, 16-1, 16-3, 16-4, 16-5, and 16-6 derived from transgenic line 1016 (R0, Lane11); Lanes 11–14: four independent R0 transgenic lines, 1016, 1001, 1017, and 1026; Lane 15: undigested genomic DNA; Lanes 16–19, undigested genomic DNAs from four independent R0 transgenic lines corresponding to Lanes 11–14. DNAs were electrophoresed in 1.0% agarose gel, transferred onto Hybond-NX membranes (Amersham), and hybridized with ³² P-labeled 0.45-kb SmaI fragment of PAT63