Fig. 3 Analysis of inositol phosphates in wild-type and lpa1-1rice seed. Standards or samples were loaded onto a strong-anion exchange column and eluted with a complex gradient. Ins Ps were detected following post-column derivitization via negative absorbance at 550 nm. (A) Elution of 100 nmol of myo-inositol 1,2,3,4,5,6-hexakisphosphate (Ins P₆) standard. (B, C) Equal amounts of wild-type (B) or lpa1-1 (C) seed tissue were extracted, and equal aliquots of filtered supernatant were fractionated as in (A)