Fig. 1 Segregation of Orion M2-70 in the M2 and M3 generations. Seeds produced by a given plant were individually crushed and assayed for inorganic P. (A) M2 generation; 10 M3 seeds were tested for each of 10 Orion M2-70 M2 plants. (B) M3 generation; 10 M4 seeds were tested for each of 14 Orion M2-70 M3 plants. Standards: (1) 0.0 µg P; (2) 0.15 µg P; (3) 0.46 µg P; (4) 0.93 µg P; (5) 1.39 µg P. In this study, wild-type rice seeds typically have inorganic P levels ( = 0.15 mg PI g-1) that yield an assay result < Standard No. 2. Orion M2-70 seeds have inorganic P levels resulting in an assay = Standard No. 5, indicating these seeds contained > 1.5 mg Pi g-1