Fig. 4 Southern hybridization of the HindIII- or EcoRI-digested genomic DNA of Lolium perenne (Lp), Festuca mairei (Fm), and their hybrid derivatives with (A) TF109, identifying the presence of F. mairei genome in F₁, F₂, and BC₁ plants; (B) TF515, a Festuca genome-specific probe, showing strong hybridization only in F₁, BC₁, and some BC₁G₁ plants (M = labeled DNA/HindIII fragments)